• Laser-Activated Microfluidic S...

Treffer: Laser-Activated Microfluidic SERS Substrates.

Title:
Laser-Activated Microfluidic SERS Substrates.
Authors:
Shestopalova MS; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.; Moscow Engineering Physics Institute, National Research Nuclear University MEPHI, Moscow 115409, Russia., Korzhov DS; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.; Moscow Engineering Physics Institute, National Research Nuclear University MEPHI, Moscow 115409, Russia., Afanasyev KN; Institute for Theoretical and Applied Electromagnetics of the Russian Academy of Sciences, Moscow 125412, Russia., Ivanov A; Institute for Theoretical and Applied Electromagnetics of the Russian Academy of Sciences, Moscow 125412, Russia., Bykov IV; Institute for Theoretical and Applied Electromagnetics of the Russian Academy of Sciences, Moscow 125412, Russia., Sarychev AK; Institute for Theoretical and Applied Electromagnetics of the Russian Academy of Sciences, Moscow 125412, Russia., Basmanov DV; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow 119435, Russia., Il'in AI; Institute of Microelectronics Technology and High Purity Materials of the Russian Academy of Sciences, Chernogolovka, Moscow Region 142432, Russia., Mochalov K; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.; RUDN University,Moscow 117198, Russia.
Source:
ACS sensors [ACS Sens] 2026 Jan 23; Vol. 11 (1), pp. 589-598. Date of Electronic Publication: 2025 Dec 22.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: American Chemical Society Country of Publication: United States NLM ID: 101669031 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2379-3694 (Electronic) Linking ISSN: 23793694 NLM ISO Abbreviation: ACS Sens Subsets: MEDLINE
Imprint Name(s):
Original Publication: Washington, DC : American Chemical Society, [2016]-
MeSH Terms:
Spectrum Analysis, Raman*/methods , Spectrum Analysis, Raman*/instrumentation , Lasers* , Microfluidic Analytical Techniques*/instrumentation , Microfluidic Analytical Techniques*/methods, Humans ; HEK293 Cells ; Silver/chemistry ; Extracellular Vesicles/chemistry
Contributed Indexing:
Keywords: Kelvin probe force microscopy; atomic force microscopy; extracellular vesicles; lab-on-chip technology; limit of detection; plasmonic nanostructures; surface-enhanced Raman spectroscopy
Substance Nomenclature:
3M4G523W1G (Silver)
Entry Date(s):
Date Created: 20251222 Date Completed: 20260123 Latest Revision: 20260123
Update Code:
20260123
DOI:
10.1021/acssensors.5c03447
PMID:
41427527
Database:
MEDLINE

Weitere Informationen

The development of automated microfluidic systems for ultrasensitive detection of biomaterials via surface-enhanced Raman spectroscopy (SERS) represents one of the most promising areas in current research. Within this field, special attention is directed toward SERS-based detection and analysis of extracellular vesicles, aimed at identifying disease biomarkers either in the form of microRNA and mRNA or membrane-bound proteins. However, practical applications of SERS detection systems, particularly those employing silver-based SERS substrates, are significantly limited due to their temporal instability caused by surface contamination and oxidation. In this work, we propose a fabrication method and operational concept for SERS substrates that are activated immediately prior to experimentation, serving as a basis for integration into chip-based spectral recording chambers within automated microfluidic systems. The nonactivated SERS substrates described herein can be embedded into microfluidic chips and stored for extended periods without loss of functionality, being activated just before the start of experimental procedures. A theoretical model was developed to evaluate local electromagnetic field enhancement in such SERS substrates. The sensitivity of these substrates was determined experimentally, demonstrating the feasibility of rapid detection of individual extracellular vesicles from HEK293T cells as well as their clusters.