Treffer: Lab on an end: Micromanipulation using the acoustohydrodynamic pillar array as an end effector.

Title:
Lab on an end: Micromanipulation using the acoustohydrodynamic pillar array as an end effector.
Authors:
Chen Z; Key Laboratory of Biomimetic Robots and Systems, Ministry of Education, State Key Laboratory of Intelligent Control and Decision of Complex System, and School of Mechatronics Engineering, Beijing Institute of Technology, Beijing 100081, China., Bai C; Key Laboratory of Biomimetic Robots and Systems, Ministry of Education, State Key Laboratory of Intelligent Control and Decision of Complex System, and School of Mechatronics Engineering, Beijing Institute of Technology, Beijing 100081, China., Liu F; Key Laboratory of Biomimetic Robots and Systems, Ministry of Education, State Key Laboratory of Intelligent Control and Decision of Complex System, and School of Mechatronics Engineering, Beijing Institute of Technology, Beijing 100081, China., Huang Q; Key Laboratory of Biomimetic Robots and Systems, Ministry of Education, State Key Laboratory of Intelligent Control and Decision of Complex System, and School of Mechatronics Engineering, Beijing Institute of Technology, Beijing 100081, China., Fukuda T; Institute of Innovation for Future Society, Nagoya University, Nagoya 4648601, Japan., Arai T; Key Laboratory of Biomimetic Robots and Systems, Ministry of Education, State Key Laboratory of Intelligent Control and Decision of Complex System, and School of Mechatronics Engineering, Beijing Institute of Technology, Beijing 100081, China.; Center for Neuroscience and Biomedical Engineering, The University of Electro-Communications, Tokyo 1828585, Japan., Liu X; Key Laboratory of Biomimetic Robots and Systems, Ministry of Education, State Key Laboratory of Intelligent Control and Decision of Complex System, and School of Mechatronics Engineering, Beijing Institute of Technology, Beijing 100081, China.
Source:
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2025 Dec 23; Vol. 122 (51), pp. e2505479122. Date of Electronic Publication: 2025 Dec 17.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE
Imprint Name(s):
Original Publication: Washington, DC : National Academy of Sciences
MeSH Terms:
Microfluidics*/methods , Microfluidics*/instrumentation , Micromanipulation*/methods , Micromanipulation*/instrumentation , Acoustics*/instrumentation , Lab-On-A-Chip Devices* , Microfluidic Analytical Techniques*/instrumentation , Microfluidic Analytical Techniques*/methods, Animals ; Caenorhabditis elegans
Grant Information:
62273052 MOST | National Natural Science Foundation of China (NSFC); W2431050 MOST | National Natural Science Foundation of China (NSFC); L248102 åŒ-京å¸'ç§'å¦æŠ€æœ¯å§"å'˜ä¼š | Natural Science Foundation of Beijing Municipality (åŒ-京å¸'自ç¶ç§'å¦åŸºé‡'); IS23062 åŒ-京å¸'ç§'å¦æŠ€æœ¯å§"å'˜ä¼š | Natural Science Foundation of Beijing Municipality (åŒ-京å¸'自ç¶ç§'å¦åŸºé‡'); 23K22712 Ministry of Education, Culture, Sports, Science and Technology (MEXT)
Contributed Indexing:
Keywords: acoustohydrodynamics; droplet manipulation; micromanipulation; open microfluidic devices; single-cell operation
Entry Date(s):
Date Created: 20251217 Date Completed: 20251217 Latest Revision: 20251231
Update Code:
20251231
PubMed Central ID:
PMC12745804
DOI:
10.1073/pnas.2505479122
PMID:
41405859
Database:
MEDLINE

Weitere Informationen

The concept of microfluidics has shown considerable promise for advancing chemistry, diagnostics, and biology. However, there have been no guaranteed routes of microfluidics that can achieve widespread adoption in mainstream chemistry and biomedical research. Inspired by the cooperative multiple cilia of biological organisms and tissues to generate flow, we propose an open microfluidic platform, lab on an end (LoE), to pump spatially and temporally continuous flow for multifunctional micromanipulation with the acoustohydrodynamic pillar array as an end effector. LoE brings together the micromanipulation of individual entities, liquid operations, and cell processing onto an acoustic end effector. These operations are mainly driven by acoustic radiation and two unique frequency-dependent microstreaming profiles: out-of-plane vortex near a single pillar and in-plane transmission flow surrounding the entire pillar array. Applications in embryo engineering, local morphological phenotyping of Caenorhabditis elegans for neuron research, efficient chemical reactions, and multifunctional cell processing indicate that the LoE could potentially lead to breakthroughs in understanding and using microfluidics. Its capability of integrating multiple sequential processes, inherent high accessibility, easy use, and low cost provide an end-to-end solution to mainstream chemistry and biomedical research.

Competing interests statement:The authors declare no competing interest.