Treffer: TaxaPLN: a taxonomy-aware augmentation strategy for microbiome-trait classification including metadata.

Background: The gut microbiome plays a crucial role in human health, making it a cornerstone of modern biomedical research. To study its structure and dynamics, machine learning models are increasingly used to identify key microbial patterns associated with disease and environmental factors, but their performance is often limited by the intrinsic complexity of microbiome data and the small size of available cohorts. In this context, data augmentation has emerged as a promising strategy to overcome these challenges by generating artificial microbiome profiles.
Results: We introduce TaxaPLN, a data augmentation method based on PLN-Tree generative models, which leverages the taxonomy and a data-driven sampler to generate realistic synthetic microbiome compositions. Additionally, we propose a conditional extension based on feature-wise linear modulation, enabling covariate-aware generation. Experiments on diverse curated microbiome datasets show that TaxaPLN preserves ecological properties and generally improves or maintains predictive performances, outperforming state-of-the-art baselines on most tasks. Furthermore, the conditional variant of TaxaPLN establishes a new benchmark for metadata-aware microbiome augmentation.
Conclusion: TaxaPLN provides a model-based framework for augmenting microbiome datasets while preserving their ecological and clinical relevance. By integrating taxonomic structure and host metadata, it enhances predictive modeling across diverse real-world settings. To facilitate reproducible and scalable microbiome analysis using our method, TaxaPLN is released as an open-source Python package available on PyPI (plntree), with MIT-licensed source code hosted at https://github.com/AlexandreChaussard/PLNTree-package .
(© 2025. The Author(s).)

Declarations. Ethics approval and consent to participate: Microbiome data used in this study originate from the publicly available curatedMetagenomicData database, which aggregates datasets approved by the respective institutional review boards. No additional ethics approval was required for our work. Consent for publication: Not applicable. Conflict of interest: The authors declare no conflict of interest.