Treffer: Drosophila video-assisted activity monitor (DrosoVAM): a versatile method for behaviour monitoring.

Title:
Drosophila video-assisted activity monitor (DrosoVAM): a versatile method for behaviour monitoring.
Authors:
Revel M; Department of Genetics and Development, University of Geneva, Geneva, Switzerland., Nagoshi E; Department of Genetics and Development, University of Geneva, Geneva, Switzerland., Maeda R; Department of Genetics and Development, University of Geneva, Geneva, Switzerland.
Source:
Royal Society open science [R Soc Open Sci] 2025 Sep 04; Vol. 12 (9), pp. 250764. Date of Electronic Publication: 2025 Sep 04 (Print Publication: 2025).
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Royal Society Publishing Country of Publication: England NLM ID: 101647528 Publication Model: eCollection Cited Medium: Print ISSN: 2054-5703 (Print) Linking ISSN: 20545703 NLM ISO Abbreviation: R Soc Open Sci Subsets: In Process
Imprint Name(s):
Original Publication: London : Royal Society Publishing, 2014-
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Contributed Indexing:
Keywords: Drosophila; behaviour; circadian rhythm; method
Molecular Sequence:
figshare 10.6084/m9.figshare.c.7979944
Entry Date(s):
Date Created: 20250908 Latest Revision: 20250910
Update Code:
20250910
PubMed Central ID:
PMC12410964
DOI:
10.1098/rsos.250764
PMID:
40919370
Database:
MEDLINE

Weitere Informationen

Drosophila melanogaster has been a pioneering model system for investigations into the genetic bases of behaviour. Studies of circadian activity were some of the first behaviours investigated in flies. The Drosophila Activity Monitoring (DAM) system by TriKinetics played a key role in establishing the fundamental feedback loop of the circadian clock. Although this method has many times proven to be extremely useful, it suffers from its simplification of activity to the interruption of an infrared (IR) beam. It is blind to fly movements not disrupting the beam and any modifications to this assay to achieve better resolution often requires the purchase of new and expensive modules. We required a relatively high-throughput system to explore the potential post-mating activity changes of larger Drosophila species. Rather than investing in a larger and more complex DAM system, we designed a new monitoring system that is more versatile, economic and sensitive than DAM. This new system, called DrosoVAM ( Drosophila Video-assisted Activity Monitoring), is simple to implement and cost efficient, using a Raspberry Pi-controlled IR, digital video system to record multiple chambers and Python scripts that drive the deep learning software DeepLabCut, to track fly activity over multiple days.
(© 2025 The Authors.)

We declare we have no competing interests.