Treffer: Evaluation of an internet of things device for isothermal molecular detection.

Title:
Evaluation of an internet of things device for isothermal molecular detection.
Authors:
Weidmann M; Midge Medical GmbH, Berlin, Germany. manfred_w.weidmann@uni-leipzig.de.; Institute of Animal Hygiene and Veterinary Public Health, Leipzig University, An den Tierkliniken 43, D-04103, Leipzig, Germany. manfred_w.weidmann@uni-leipzig.de., Alvarez JB; Midge Medical GmbH, Berlin, Germany., Zinn S; Midge Medical GmbH, Berlin, Germany., Ibragimov R; Midge Medical GmbH, Berlin, Germany., Hasani M; Midge Medical GmbH, Berlin, Germany., Graf E; Midge Medical GmbH, Berlin, Germany., Weber A; Midge Medical GmbH, Berlin, Germany., Arrey F; Midge Medical GmbH, Berlin, Germany., Szczygiel MM; Midge Medical GmbH, Berlin, Germany., Kowalewska Z; Midge Medical GmbH, Berlin, Germany., Kobialka RM; Institute of Animal Hygiene and Veterinary Public Health, Leipzig University, An den Tierkliniken 43, D-04103, Leipzig, Germany., Ceruti A; Institute of Animal Hygiene and Veterinary Public Health, Leipzig University, An den Tierkliniken 43, D-04103, Leipzig, Germany., Wahed AAE; Institute of Animal Hygiene and Veterinary Public Health, Leipzig University, An den Tierkliniken 43, D-04103, Leipzig, Germany., Diebold M; Midge Medical GmbH, Berlin, Germany., Wahnes C; Midge Medical GmbH, Berlin, Germany.
Source:
Infection [Infection] 2025 Dec; Vol. 53 (6), pp. 2467-2480. Date of Electronic Publication: 2025 Jun 13.
Publication Type:
Journal Article; Evaluation Study
Language:
English
Journal Info:
Publisher: Springer Heidelberg Country of Publication: Germany NLM ID: 0365307 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1439-0973 (Electronic) Linking ISSN: 03008126 NLM ISO Abbreviation: Infection Subsets: MEDLINE
Imprint Name(s):
Publication: 2011- : Heidelberg : Springer Heidelberg
Original Publication: München, Verlagsgesellschaft Otto Spatz.
MeSH Terms:
COVID-19*/diagnosis , Molecular Diagnostic Techniques*/instrumentation , Molecular Diagnostic Techniques*/methods , SARS-CoV-2*/genetics , SARS-CoV-2*/isolation & purification , Nucleic Acid Amplification Techniques*/instrumentation , Nucleic Acid Amplification Techniques*/methods , Internet of Things*, Humans ; Sensitivity and Specificity ; Smartphone
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Contributed Indexing:
Keywords: Home test; Internet of things; Point of care test; Recombinase polymerase amplification; SARS-CoV-2
SCR Protocol:
LAMP assay
Entry Date(s):
Date Created: 20250613 Date Completed: 20251203 Latest Revision: 20251206
Update Code:
20251206
PubMed Central ID:
PMC12675774
DOI:
10.1007/s15010-025-02581-1
PMID:
40512363
Database:
MEDLINE

Weitere Informationen

We describe a low-cost up scalable molecular test platform providing accessibility to frequent testing - the prerequisite to control infectious epidemics. The device uses the Multi-Spectral Digital Sensor AS7341 in a small fluorescence spectrometer which communicates with a Smartphone App via Bluetooth, which passes on the raw data to outsourced computation in the cloud via the internet. This very stable setup allows easy to use on site testing of individual samples and anonymised data analysis while providing the basis for direct result transmission of a rapid isothermal molecular diagnostic test result not only to the user but also to public health bodies. This internet of things (IoT) platform allows to collect real molecular test data from users with usability and industrial up scalability at the heart of the design. The device characteristics are described in detail and the performance for a rapid SARS-CoV-2 Recombinase Polymerase Amplification assay achieved 98.6% sensitivity and 98% specificity testing 148 positive and 501 negative samples.
(© 2025. The Author(s).)

Declarations. Competing interests: The authors declare no competing interests.