• Pseudo-color-assisted aggregat...

Treffer: Pseudo-color-assisted aggregation-induced emission dye biosensor for "label-free" monitoring of ochratoxin A signals in plants.

Title:
Pseudo-color-assisted aggregation-induced emission dye biosensor for "label-free" monitoring of ochratoxin A signals in plants.
Authors:
Wu M; College of Life Sciences, Ludong University, Yantai 264025, PR China., Li S; School of Chemistry, Chemical Engineering and Life Sciences (Aix-Marseille Institute), Wuhan University of Technology, Wuhan 430070, PR China., Wu Q; Dongping County Pengji Street Agricultural Comprehensive Service Center, Tai'an 271509, PR China., Hu M; College of Life Sciences, Ludong University, Yantai 264025, PR China., Huang L; College of Life Sciences, Ludong University, Yantai 264025, PR China., Li X; College of Life Sciences, Ludong University, Yantai 264025, PR China. Electronic address: xiangyang_l@163.com.
Source:
Journal of hazardous materials [J Hazard Mater] 2025 Aug 15; Vol. 494, pp. 138770. Date of Electronic Publication: 2025 May 28.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9422688 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3336 (Electronic) Linking ISSN: 03043894 NLM ISO Abbreviation: J Hazard Mater Subsets: MEDLINE
Imprint Name(s):
Original Publication: Amsterdam : Elsevier,
MeSH Terms:
Ochratoxins*/analysis , Biosensing Techniques*/methods , Fluorescent Dyes*/chemistry, Aptamers, Nucleotide/chemistry ; Limit of Detection
Contributed Indexing:
Keywords: Aptamers; Economic crops; Fluorescence imaging; Fungal toxins; Pseudo-color
Substance Nomenclature:
0 (Ochratoxins)
1779SX6LUY (ochratoxin A)
0 (Fluorescent Dyes)
0 (Aptamers, Nucleotide)
Entry Date(s):
Date Created: 20250601 Date Completed: 20250701 Latest Revision: 20250701
Update Code:
20250701
DOI:
10.1016/j.jhazmat.2025.138770
PMID:
40451007
Database:
MEDLINE

Weitere Informationen

Ochratoxin A (OTA) is a fungal toxin that is highly toxic and widely distributed, causing serious damage to important economic crops. Understanding their presence in plants can help protect economic crops from infection. In this work, a "label-free" OTA sensing probe that directly uses aptamer sequences to achieve targeted response was prepared. The aptamer hybridized with cDNA can resist digestion by exonuclease (EXO.I), while the presence of OTA competes with the aptamer and disrupts the resistance process. The entire sensing process can be monitored by the fluorescent indicator aggregation-induced emission dye (QAFI). The sensing method has a detection limit of 17 pM and a linear range of 0.05-150 nM. This fluorescent probe can not only perform on-site analysis of OTA in vitro, but can also be easily incubated into plants for fluorescence monitoring. More importantly, in view of the problem that the single-color presentation of traditional fluorescence images is not easy to observe, this work uses Java algorithms to develop a pseudo-color one-key conversion applet. This greatly saves researchers time in analyzing images. On this basis, this work explores the characteristics of OTA infection in plants under various physiological states, which has a certain reference significance for the planting and cultivation of crops.
(Copyright © 2025. Published by Elsevier B.V.)

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.